We conducted road surveys of Puerto Rican Screech-Owls (Otus nudipes) by playing conspecific vocalizations in secondary wet forest and fragmented secondary moist forest in rural areas of eastern Puerto Rico. Six paired surveys were conducted bi-weekly beginning in April. We recorded number of owl responses, cloud cover, wind speed, moon phase, and number of passing cars during 5-min stops at 60 locations. Owls responded in similar numbers (P > 0.05) in both habitat types. Also, we detected no association with cloud cover, wind speed, moon phase, or passing cars.