thumbnail

Hepatic microsomal cytochromes P450 in mink fed Saginaw Bay carp (SBC)

Society of Environmental Toxicology and Chemistry, annual meeting abstract book
By: , and 

Links

  • The Publications Warehouse does not have links to digital versions of this publication at this time
  • Download citation as: RIS | Dublin Core

Abstract

Livers from mink fed diets containing 0% (n = 12), 10% (n = 11), 20% (n = 12) and 40% (n = 10) SBC for 6 months contained 0.1, 2.2, 3.6, and 6.3 ug/g total PCBs, respectively. Hepatic microsomes were prepared and assayed for protein, arylhydrocarbon hydroxylase (AHH), benzyloxyresorufin-O-dealkylase (BROD), ethoxy-ROD (ER0D), pentoxy-ROD (PROD), and ethoxycoumarin-OD (ECOD). Mink fed SBC had increased AHH, EROD, and ECOD (group means 2.2-3.4 X control means), decreased BROD and unchanged PROD (the latter 2 assays indicators for phenobarbital-type induction in mammals). Three samples from each group were examined by western blot using a polyclonal anti-P450llB antibody and a monoclonal anti-P450lA antibody (MAb 1-12-3). Mink fed SBC showed induction of a protein recognized by anti-P450lA (8 X control), but had little protein recognized by anti-P450IlB. The monooxygenase activities and western blot data give a consistent picture of MC-type but not PB-type induction in mink fed SBC.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Hepatic microsomal cytochromes P450 in mink fed Saginaw Bay carp (SBC)
Series title Society of Environmental Toxicology and Chemistry, annual meeting abstract book
Volume 13
Year Published 1992
Language English
Contributing office(s) Patuxent Wildlife Research Center
Description 197 (abstract no. TA6F23)
Larger Work Type Article
Larger Work Subtype Journal Article
Larger Work Title Society of Environmental Toxicology and Chemistry, annual meeting abstract book
First page 197 (abstr