Oxidative stress induced in PCB 126-exposed northern leopard frogs, Rana pipiens

Journal of Toxicology and Environmental Health, Part A
6727_Huang.pdf
By: , and 

Links

Abstract

Northern leopard frogs Rana pipiens exposed to PCB 126 (3,3',4,4',5-pentachlorobiphenyl) were examined for hepatic oxidative stress. In a dose-response study, northern leopard frogs were injected intraperitoneally with either PCB 126 in corn oil (0.2, 0.7, 2.3, or 7.8 mg/kg body weight) or corn oil alone. In a time-course study, frogs received 7.8 mg/kg or corn oil alone, and were examined at 1, 2, 3, and 4 wk after dosing. Hepatic concentrations of reduced glutathione (GSH), thiobarbituric acid-reactive substances (TBARS), and total sulfhydryls (total SH), as well as activities of glutathione peroxidase (GSH-P), GSSG reductase (GSSG-R), glucose-6-phosphate dehydrogenase (G-6-PDH), and glutathione S-transferase (GSH-S-T) were measured. In the dose-response experiment, few effects were apparent 1 wk after dosing. In the time-course experiment, significant changes were observed in the 7.8-mg/kg group at 2 wk or more posttreatment. Hepatic concentrations of GSH and TBARS were higher than in corresponding controls at wk 3 and 4; the activities of GSSG-R and GSH-S-T were higher than in controls at wk 2 and 4; and the activity of G-6-PDH was increased at wk 2 and 4. These data collectively indicate that altered glutathione metabolism and oxidative stress occurred and were indicative of both toxicity and induction of protective mechanisms in frogs exposed to PCB. A similar delay in response was reported in fish and may relate to lower metabolic rate and physiological reactions in ectothermic vertebrates

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Oxidative stress induced in PCB 126-exposed northern leopard frogs, Rana pipiens
Series title Journal of Toxicology and Environmental Health, Part A
DOI 10.1080/15287390600974676
Volume 70
Issue 8
Year Published 2007
Language English
Contributing office(s) Patuxent Wildlife Research Center
Description 676-681
Larger Work Type Article
Larger Work Subtype Journal Article
Larger Work Title Journal of Toxicology and Environmental Health, Part A
First page 676
Last page 681