A noninvasive, direct real-time PCR method for sex determination in multiple avian species

Molecular Ecology Resources
By: , and 

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Abstract

Polymerase chain reaction (PCR)-based methods to determine the sex of birds are well established and have seen few modifications since they were first introduced in the 1990s. Although these methods allowed for sex determination in species that were previously difficult to analyse, they were not conducive to high-throughput analysis because of the laboriousness of DNA extraction and gel electrophoresis. We developed a high-throughput real-time PCR-based method for analysis of sex in birds, which uses noninvasive sample collection and avoids DNA extraction and gel electrophoresis.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title A noninvasive, direct real-time PCR method for sex determination in multiple avian species
Series title Molecular Ecology Resources
DOI 10.1111/j.1755-0998.2010.02951.x
Volume 11
Issue 2
Year Published 2011
Language English
Publisher Wiley-Blackwell
Publisher location Malden, MA
Contributing office(s) Patuxent Wildlife Research Center, Contaminant Biology Program
Description 3 p.
First page 415
Last page 417