Characterization of culturable bacteria isolated from the cold-water coral Lophelia pertusa

FEMS Microbiology Ecology
By: , and 

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Abstract

Microorganisms associated with corals are hypothesized to contribute to the function of the host animal by cycling nutrients, breaking down carbon sources, fixing nitrogen, and producing antibiotics. This is the first study to culture and characterize bacteria from Lophelia pertusa, a cold-water coral found in the deep sea, in an effort to understand the roles that the microorganisms play in the coral microbial community. Two sites in the northern Gulf of Mexico were sampled over 2 years. Bacteria were cultured from coral tissue, skeleton, and mucus, identified by 16S rRNA genes, and subjected to biochemical testing. Most isolates were members of the Gammaproteobacteria, although there was one isolate each from the Betaproteobacteria and Actinobacteria. Phylogenetic results showed that both sampling sites shared closely related isolates (e.g. Pseudoalteromonas spp.), indicating possible temporally and geographically stable bacterial-coral associations. The Kirby-Bauer antibiotic susceptibility test was used to separate bacteria to the strain level, with the results showing that isolates that were phylogenetically tightly grouped had varying responses to antibiotics. These results support the conclusion that phylogenetic placement cannot predict strain-level differences and further highlight the need for culture-based experiments to supplement culture-independent studies.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Characterization of culturable bacteria isolated from the cold-water coral Lophelia pertusa
Series title FEMS Microbiology Ecology
Volume 77
Issue 2
Year Published 2011
Language English
Publisher Wiley
Publisher location Hoboken, NJ
Contributing office(s) St. Petersburg Coastal and Marine Science Center
Description 14 p.
Larger Work Type Article
Larger Work Subtype Journal Article
Larger Work Title FEMS Microbiology Ecology
First page 333
Last page 346