Review of concepts useful for maintaining quality of male reproductive field samples for laboratory study

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Abstract

Investigations into cellular and molecular characteristics of male gametes obtained from fish in natural ecosystems require careful sample handling and shipping in order to minimize artifacts. Maintaining sample integrity engenders confident assessments of ecosystem health, whereby animal condition is often reflected by gamete biomarkers - indicators that respond in measurable ways to changes. A number of our investigations have addressed the hypothesis that biomarkers from fish along a pollution gradient are reflective of site location. Species biology and the selected biological endpoints direct choice of parameters such as: temperature, buffer osmolality, time in transit, fixation, cryoprotectants, protease inhibition, and antibiotic inclusion in extender. This paper will highlight case studies, and outline parameters and thoughts on approaches for use by field and laboratory researchers.

Additional publication details

Publication type Conference Paper
Publication Subtype Conference Paper
Title Review of concepts useful for maintaining quality of male reproductive field samples for laboratory study
Year Published 2011
Language English
Publisher Diamond Congress Ltd.
Publisher location Budapest, Hungary
Contributing office(s) National Wetlands Research Center
Description 3 p.
Larger Work Type Book
Larger Work Subtype Conference publication
Larger Work Title 3rd International Workshop on the Biology of Fish Gametes, Budapest, Hungary, 7-9 September 2011
First page 75
Last page 77