Detecting contaminant-induced apoptosis and necrosis in lake trout thymocytes via flow cytometry.
This chapter details the cytofluorometric techniques employed to assess levels of active (apoptosis) and passive (necrotic) cell death in untreated and contaminant-treated fish thymocytes. The thymus is believed to be a central component of hematopoiesis and immune function in teleosts (Abelli et al., 1996). Hence, chemically-elicited adverse effects to the thymus may result in immunomodulation and organ dysfunction. However, it is not well documented that environmental contaminants induce apoptosis, or programmed cell death. There is some evidence suggesting that low level exposure to waterborne contaminants can specifically induce cell death in the olfactory epithelium of rainbow trout (Julliard et al., 1996). Presently, only limited information is available in the literature regarding apoptotic death in piscine immune cells (Alford et al., 1994; Greenlee et al., 1991).
|Publication type||Book chapter|
|Publication Subtype||Book Chapter|
|Title||Detecting contaminant-induced apoptosis and necrosis in lake trout thymocytes via flow cytometry.|
|Publisher location||Fair Haven, NJ|
|Contributing office(s)||Great Lakes Science Center|
|Larger Work Type||Book|
|Larger Work Subtype||Other Government Series|
|Larger Work Title||Techniques in Fish Immunology-3|
|Google Analytics Metrics||Metrics page|