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Detecting contaminant-induced apoptosis and necrosis in lake trout thymocytes via flow cytometry.

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By: , and 
Edited by: J.S. StolenT.C. FletcherA.F. RowleyJ.T. ZelikoffS.L. Kaattari, and S.A. Smith

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Abstract

This chapter details the cytofluorometric techniques employed to assess levels of active (apoptosis) and passive (necrotic) cell death in untreated and contaminant-treated fish thymocytes. The thymus is believed to be a central component of hematopoiesis and immune function in teleosts (Abelli et al., 1996). Hence, chemically-elicited adverse effects to the thymus may result in immunomodulation and organ dysfunction. However, it is not well documented that environmental contaminants induce apoptosis, or programmed cell death. There is some evidence suggesting that low level exposure to waterborne contaminants can specifically induce cell death in the olfactory epithelium of rainbow trout (Julliard et al., 1996). Presently, only limited information is available in the literature regarding apoptotic death in piscine immune cells (Alford et al., 1994; Greenlee et al., 1991).

Additional publication details

Publication type Book chapter
Publication Subtype Book Chapter
Title Detecting contaminant-induced apoptosis and necrosis in lake trout thymocytes via flow cytometry.
Edition 2nd
Year Published 1997
Language English
Publisher SOS Publications
Publisher location Fair Haven, NJ
Contributing office(s) Great Lakes Science Center
Description p. 203-208
Larger Work Type Book
Larger Work Subtype Other Government Series
Larger Work Title Techniques in Fish Immunology-3
First page 203
Last page 208
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