Allantoinase in lake trout (Salvelinus namaycush): In vitro effects of PCBs, DDT and metals

Comparative Biochemistry and Physiology, Part C: Comparative Pharmacology
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Abstract

1. Allantoinase, an enzyme in the purine-urea cycle, was found in livers of Salvelinus namaycush (Osteichthyes: Salmoniformes).

2. The enzyme was active from pH 6.6 to 8.2 at 37°C and from pH 7.4 to 9.0 at 10°C and had an Arrhenius energy was activation of 11.0 kcal/mol and a temperature quotient of 2.0. The Km of the enzyme homogenate was 8.4 mM allantoin.

3. The concentrations of inorganic metals at which 50% inhibition occurred during in vitro exposure were 6.0 mg/l Cu2+, 6.7 mg/l Cd2+, 34 mg/l Hg2+ and 52 mg/l Pb2+. The in vitro sensitivity to PCBs, DDT and DDE and kinetics in the presence of metals were determined.

4. Allantoinase activity was negatively correlated with body length for fish from Lake Michigan but not from Lake Superior or the laboratory.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Allantoinase in lake trout (Salvelinus namaycush): In vitro effects of PCBs, DDT and metals
Series title Comparative Biochemistry and Physiology, Part C: Comparative Pharmacology
DOI 10.1016/0306-4492(79)90102-3
Volume 62
Issue 1
Year Published 1979
Language English
Publisher Elsevier
Contributing office(s) Great Lakes Science Center
Description 5 p.
First page 71
Last page 75