A new selective enrichment procedure for isolating Pasteurella multocida from avian and environmental samples

Avian Diseases
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Abstract

A selective enrichment procedure, using two new selective media, was developed to isolate Pasteurella multocida from wild birds and environmental samples. These media were developed by testing 15 selective agents with six isolates of P. multocida from wild avian origin and seven other bacteria representing genera frequently found in environmental and avian samples. The resulting media—Pasteurella multocida selective enrichment broth and Pasteurella multocida selective agar—consisted of a blood agar medium at pH 10 containing gentamicin, potassium tellurite, and amphotericin B. Media were tested to determine: 1) selectivity when attempting isolation from pond water and avian carcasses, 2) sensitivity for detection of low numbers of P. multocida from pure and mixed cultures, 3) host range specificity of the media, and 4) performance compared with standard blood agar. With the new selective enrichment procedure, P. multocida was isolated from inoculated (60 organisms/ml) pond water 84% of the time, whereas when standard blood agar was used, the recovery rate was 0%.

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Additional publication details

Publication type Article
Publication Subtype Journal Article
Title A new selective enrichment procedure for isolating Pasteurella multocida from avian and environmental samples
Series title Avian Diseases
DOI 10.2307/1591956
Volume 38
Issue 2
Year Published 1994
Language English
Publisher American Association of Avian Pathologists
Publisher location Jacksonville, FL
Contributing office(s) National Wildlife Health Center
Description 8 p.
First page 317
Last page 324
Country United States
State California, Illinois, Indiana, Iowa, Maryland, Massachusetts, Minnesota, Mississippi, Nebraska, New Mexico, New York, South Carolina, Texas, Virginia, West Virginia, Wisconsin, Wyoming
Online Only (Y/N) N
Additional Online Files (Y/N) N
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