Characterization of low-pathogenicity H5N1 avian influenza viruses from North America

Journal of Virology
Abstract has subscript/superscript to be fixed
By: , and 



Wild-bird surveillance in North America for avian influenza (AI) viruses with a goal of early identification of the Asian H5N1 highly pathogenic AI virus has identified at least six low-pathogenicity H5N1 AI viruses between 2004 and 2006. The hemagglutinin (HA) and neuraminidase (NA) genes from all 6 H5N1 viruses and an additional 38 North American wild-bird-origin H5 subtype and 28 N1 subtype viruses were sequenced and compared with sequences available in GenBank by phylogenetic analysis. Both HA and NA were phylogenetically distinct from those for viruses from outside of North America and from those for viruses recovered from mammals. Four of the H5N1 AI viruses were characterized as low pathogenicity by standard in vivo pathotyping tests. One of the H5N1 viruses, A/MuteSwan/MI/451072-2/06, was shown to replicate to low titers in chickens, turkeys, and ducks. However, transmission of A/MuteSwan/MI/451072-2/06 was more efficient among ducks than among chickens or turkeys based on virus shed. The 50% chicken infectious dose for A/MuteSwan/MI/451072-2/06 and three other wild-waterfowl-origin H5 viruses were also determined and were between 105.3 and 107.5 50% egg infective doses. Finally, seven H5 viruses representing different phylogenetic clades were evaluated for their antigenic relatedness by hemagglutination inhibition assay, showing that the antigenic relatedness was largely associated with geographic origin. Overall, the data support the conclusion that North American H5 wild-bird-origin AI viruses are low-pathogenicity wild-bird-adapted viruses and are antigenically and genetically distinct from the highly pathogenic Asian H5N1 virus lineage.

Study Area

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Characterization of low-pathogenicity H5N1 avian influenza viruses from North America
Series title Journal of Virology
Volume 81
Issue 21
Year Published 2007
Language English
Publisher American Society for Microbiology
Publisher location Washington, D.C.
Contributing office(s) National Wildlife Health Center
Description 8 p.
Larger Work Type Article
Larger Work Subtype Journal Article
Larger Work Title Journal of Virology
First page 11612
Last page 11619
Time Range Start 2004-01-01
Time Range End 2006-12-31
Other Geospatial North America
Online Only (Y/N) N
Additional Online Files (Y/N) N