Is nothing sacred?
Fish Health Section Newsletter
By: G. L. Hoffman
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Abstract
N-sodium-N-chloro-rho-toluenesulfonamide (chloramine-T) effectively controls bacterial gill disease (BGD) in cultured fishes, BGD, a common disease of hatchery-reared salmonids, causes more fish losses than any other disease among these species, This study describes a liquid chromatographic (LC) method that is capable of direct, simultaneous analysis of chloramine-T and its primary degradation product, rho-toluenesulfonamide (rho-TSA), in water. The procedure involves reversed-phase (C-18) LC analysis with ion suppression, using 0.01 M phosphate buffer at pH 3. The mobile phase is phosphate buffer-acetonitrile (60 + 40) at 1 mL/min. Both chemicals can be detected with a UV spectrophotometer at 229 nm; the method is linear up to 40 mg, chloramine-T or rho-TSA/L. Mean recoveries were 96.4 +/- 6.1% for water samples fortified with 0.03 mg chloramine-T/L and 95.3 +/- 4.6% for water samples fortified with 0.005 mg rho-TSA/L. Limits of detection without sample enrichment for chloramine-T and rho-TSA are 0.01 mg/L and 0.001 mg/L, respectively.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Is nothing sacred? |
| Series title | Fish Health Section Newsletter |
| Volume | 8 |
| Issue | 4 |
| Year Published | 1980 |
| Language | English |
| Publisher | American Fisheries Society |
| Publisher location | Bethesda, MD |
| Contributing office(s) | Leetown Science Center |
| Description | 1 p. |
| Larger Work Type | Article |
| Larger Work Subtype | Journal Article |
| Larger Work Title | Fish Health Section Newsletter |
| First page | 3 |
| Last page | 3 |
| Google Analytic Metrics | Metrics page |