A rapid high-performance liquid-chromatographic method for simultaneously determining the concentrations of TFM and Bayer 73 in water during lampricide treatments
Canadian Journal of Fisheries and Aquatic Sciences
None
By: V. K. Dawson
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Abstract
The high-performance liquid-chromatography (HPLC) procedure requires only minutes per sample, is specific, and is relatively sensitive (limit of detection < 0.005 mg/L for both chemicals). A combined buffer (pH 4) and internal standard (3-chlorodiphenylamine) reagent is added to the water sample, which is injected through a Sep Pak C18 disposable cartridge. The cartridge adsorbs and retains both the lampricides and the internal standard. The quantitative elution of the three chemicals from the cartridge with a small volume of methanol effectively concentrates the sample and provides sample cleanup. The methanol extract is then analyzed directly by HPLC on an MCH 10 reverse phase column by using a methanol:0.01 mol/L acetate buffer (87:13, v:v) as the mobile phase at 2 mL/min and detected by ultraviolet spectrophotometry at 330 (or 254) nm. A microprocessor data system further facilitates the procedure by quantifying off-scale peaks and yielding results directly in units of concentration (mg/L).
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | A rapid high-performance liquid-chromatographic method for simultaneously determining the concentrations of TFM and Bayer 73 in water during lampricide treatments |
| Series title | Canadian Journal of Fisheries and Aquatic Sciences |
| DOI | 10.1139/f82-105 |
| Volume | 39 |
| Issue | 5 |
| Year Published | 1982 |
| Language | English |
| Publisher | NRC Research Press |
| Publisher location | Ottawa, Ontario |
| Contributing office(s) | Upper Midwest Environmental Sciences Center |
| Description | 5 p. |
| Larger Work Type | Article |
| Larger Work Subtype | Journal Article |
| Larger Work Title | Canadian Journal of Fisheries and Aquatic Sciences |
| First page | 778 |
| Last page | 782 |
| Google Analytic Metrics | Metrics page |