Tissue distribution and elimination of rotenone in rainbow trout

Aquatic Toxicology
Abstract has subscript/superscript to be fixed



The fate of a single i.v. dose (120 μg/kg) of the piscicide [14C]rotenone was evaluated in rainbow trout for periods up to 72 h after dosing. Rotenone was rapidly cleared from the plasma; less than 2% of the dose remained in the plasma compartment after 20 min. The highest concentrations of rotenone residues (% dose/g tissue) were in the hepatobiliary system, bile, intestine, and in heart, lateral line swimming muscle, and posterior kidney; tissues that are highly dependent on oxidative metabolism. Although rotenone activity was present in all cell fractions examined, greater than 40% was associated with the mitochondrial fraction of liver, kidney, and muscle. More than 85% of the activity extracted from these tissues, except the liver, was parent rotenone. Elimination from whole body and major tissue depots conformed to simple first-order kinetics; the estimated half-life from whole body was 68.5 h. Branchial elimination accounted for 5% of the injected dose over a 4-h period, and urinary elimination was less than 2% over a 48-h period. Rotenone was eliminated essentially unchanged across the gills; however, parent rotenone was not found in either urine or bile. More than 80% of the activity in both urine and bile eluted from HPLC chromatographs as a highly polar fraction that was not hydrolyzed by incubation with either β-glucuronidase or sulfatase. The results imply that hepatobiliary excretion is the major route of elimination for rotenone residues in the trout and that metabolism to a more polar form is a prerequisite for elimination in both the bile and the urine
Publication type Article
Publication Subtype Journal Article
Title Tissue distribution and elimination of rotenone in rainbow trout
Series title Aquatic Toxicology
DOI 10.1016/0166-445X(86)90070-6
Volume 8
Issue 1
Year Published 1986
Language English
Publisher Elsevier
Publisher location Amsterdam, Netherlands
Contributing office(s) Upper Midwest Environmental Sciences Center
Description 13 p.
Larger Work Type Article
Larger Work Subtype Journal Article
Larger Work Title Aquatic Toxicology
First page 27
Last page 40
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