Use of real-time PCR to detect canine parvovirus in feces of free-ranging wolves
Journal of Wildlife Diseases
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Abstract
Using real-time PCR, we tested 15 wolf (Canis lupus) feces from the Superior National Forest (SNF), Minnesota, USA, and 191 from Yellowstone National Park (YNP), USA, collected during summer and 13 during winter for canine parvovirus (CPV)-2 DNA. We also tested 20 dog feces for CPV-2 DNA. The PCR assay was 100% sensitive and specific with a minimum detection threshold of 104 50% tissue culture infective dose. Virus was detected in two winter specimens but none of the summer specimens. We suggest applying the technique more broadly especially with winter feces.
Study Area
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Use of real-time PCR to detect canine parvovirus in feces of free-ranging wolves |
| Series title | Journal of Wildlife Diseases |
| DOI | 10.7589/0090-3558-48.2.473 |
| Volume | 48 |
| Issue | 2 |
| Year Published | 2012 |
| Language | English |
| Publisher | Wildlife Disease Association |
| Contributing office(s) | Northern Prairie Wildlife Research Center |
| Description | 4 p. |
| First page | 473 |
| Last page | 476 |
| Country | United States |
| State | Minnesota, Wyoming |
| Other Geospatial | Superior National Forest, Yellowstone National Park |
| Google Analytic Metrics | Metrics page |