Isolation of anaerobic oxalate-degrading bacteria from freshwater lake sediments
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Abstract
Enrichment cultures that anaerobically degraded oxalate were obtained from lake sediment inocula. From these, 5 pure cultures of anaerobic oxalate-degrading bacteria were isolated and partially characterized. The isolates were Gram-negative, non-sporeforming, non-motile, obligate anaerobes. Oxalate was required for growth and was stoichiometrically converted to formate; 14CO2 was also recovered when 14C-oxalate was added. Maximal growth occurred when the oxalate concentration was 50 mM. Acetate stimulated growth in the presence of oxalate, however, 14C-experiments indicated that acetate was only utilized for cell carbon.
The isolates were either spiral-shaped or rod-shaped organisms. The first morphotype grew much more slowly than the second and exhibited 13-fold lower cell yields. These isolates represent a new strain of oxalate-degrading bacteria. The second morphotype was similar to the anaerobic oxalate-degrading bacteria previously found in rumen. This report extends the known habitats in which anaerobic oxalate-degrading organisms have been found to include aquatic sediments.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Isolation of anaerobic oxalate-degrading bacteria from freshwater lake sediments |
| Series title | Archives of Microbiology |
| DOI | 10.1007/BF00446732 |
| Volume | 141 |
| Issue | 1 |
| Year Published | 1985 |
| Language | English |
| Publisher | Springer |
| Contributing office(s) | Toxic Substances Hydrology Program |
| Description | 6 p. |
| First page | 8 |
| Last page | 13 |
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