Application of a hollow-fiber, tangential-flow device for sampling suspended bacteria and particles from natural waters
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Abstract
The design and application of a hollow-fiber tangential-flow filtration device has been used to concentrate bacteria and suspended particles from large volume surface water and groundwater samples (i.e., hundreds of liters). Filtrate tlux rates (4–8 L min−1) are equal to or faster than those of other devices that are based on continuous flow centrifugation and plate and frame filtration. Particle recovery efficiencies for inorganic particles (approximately 90%) were similar to other dewatering devices, but microbial cell recoveries (30–90%) were greatly improved by this technique relative to other currently available methods. Although requirements for operation and maintenance of the device are minimal, its size, as with other dewatering devices, limits its applicability at remote sample sites. Nevertheless, it has proven useful for sample collection in studies involving microbial transport and analysis of particle-associated trace inorganic solutes.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Application of a hollow-fiber, tangential-flow device for sampling suspended bacteria and particles from natural waters |
| Series title | Journal of Environmental Quality |
| DOI | 10.2134/jeq1990.00472425001900030045x |
| Volume | 19 |
| Issue | 3 |
| Year Published | 1990 |
| Language | English |
| Publisher | American Society of Agronomy, Crop Science Society of America, Soil Science Society of America |
| Publisher location | Madison, WI |
| Contributing office(s) | San Francisco Bay-Delta, Toxic Substances Hydrology Program, Pacific Regional Director's Office |
| Description | 5 p. |
| First page | 625 |
| Last page | 629 |
| Online Only (Y/N) | N |
| Additional Online Files (Y/N) | N |
| Google Analytic Metrics | Metrics page |