Temperature and formation resistivity logs obtained in borehole KP-1 of the Hawaii Scientific Drilling Project indicate that the adjacent formation is characterized by several zones of distinctly different average temperature and water salinity. A series of hydraulic analyses and water sampling programs were conducted to rule out the possibility of local hydraulic effects associated with the presence of the borehole in the generation of these apparent groundwater zones. Hydraulic tests and sampling with the borehole cased to a depth of 710 m and open below that depth indicate that the deep aquifer contains seawater at a temperature nearly identical to that of the open ocean at the same depth. Various analyses give estimates of aquifer transmissivity of about 10-3 m2/s in the vicinity of the borehole. Isolation of this deeper aquifer from the overlying groundwater zones was investigated by perforating the casing at six locations and then measuring the changes in water level in the borehole, in the salinity of the fluid column, in the temperature profile of the fluid column, and in the rate of flow in the fluid column induced by the perforations. These results positively confirm that the zones of distinctly different formation properties indicated on the temperature and resistivity logs are not caused by flow in or around casing. Flow and fluid column salinity induced by the perforations also confirm significant differences between the hydraulic heads and geochemistry of the different groundwater zones inferred from the well logs.