The mechanisms that resuspend bottom sediments in Old Tampa Bay, a shallow, microtidal, subtropical estuary in west-central Florida, were determined by analysing data collected during several periods from 1988 to 1990. Hydrodynamic and suspended-solids concentration data were collected at a relatively deep (4 m) site where a permanent platform was built and at a relatively shallow (1·5 m) site where a submersible instrument package was deployed. Bottom sediments were non-cohesive silts and fine sands. The primary sediment resuspension mechanism at both sites was wind waves, which were generated by strong and sustained winds associated with winter storms and tropical storms. At the platform, waves were depth-transitional, and estimated bottom shear stresses were most sensitive to wave period and water depth. Concentrations of suspended solids at this site corresponded well with wave motion, and non-linear wave-current interaction was small. At the shallow-water site, concentrations of suspended solids were elevated during periods of strong north-easterly winds and large bottom orbital velocities. At both sites, wind direction was an important factor in determining the occurrence and magnitude of sediment resuspension. Resuspended sediments settled within several hours as storm intensity diminished. Winds and waves generated by thunderstorms were more transient than those generated by winter storms and tropical storms. Based on the data collected during this study, thunderstorms are less likely to resuspend bottom sediment than winter storms and tropical storms. Maximum tidal currents at the study sites are usually less than 15 cm s⁻¹and did not increase observed concentrations of suspended solids.