Liquid chromatographic determination of benzocaine and N-acetylbenzocaine in the edible fillet tissue from rainbow trout

Journal of Chromatography A
By: , and 

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Abstract

A method was developed for determining benzocaine and N-acetylbenzocaine concentrations in fillet tissue of rainbow trout. The method involves extracting the analytes with acetonitrile, removing lipids or hydrophobic compounds from the extract with hexane, and providing additional clean-up with solid-phase extraction techniques. Analyte concentrations are determined using reversed-phase high-performance liquid chromatographic techniques with an isocratic mobile phase and UV detection. The accuracy (range, 92 to 121%), precision (R.S.D., <14%), and sensitivity (method quantitation limit, <24 ng/g) for each analyte indicate the usefulness of this method for studies characterizing the depletion of benzocaine residues from fish exposed to benzocaine. Copyright (C) 1999.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Liquid chromatographic determination of benzocaine and N-acetylbenzocaine in the edible fillet tissue from rainbow trout
Series title Journal of Chromatography A
DOI 10.1016/S0021-9673(99)00665-2
Volume 855
Issue 1
Year Published 1999
Language English
Larger Work Type Article
Larger Work Subtype Journal Article
Larger Work Title Journal of Chromatography A
First page 255
Last page 260