The reproductive biology, glochidial morphology and recruitment of the federally endangered shinyrayed pocketbook, Lampsilis subangulata, were studied from May 1995 to July 1996 in the Flint River system, Georgia. Gravid female L. subangulata were found nine months of the year. On 19 May 1995, a L. subangulata was discovered releasing a superconglutinate, the first record confirming that this species used this specialized reproductive strategy. Superconglutinate release occurred from late May to mid-July in water temperatures ranging from 20.0 to 23.5 C. Laboratory experiments indicated that two fish species served as primary hosts, the spotted bass (Micropterus punctulatus) and largemouth bass (M. salmoides). Secondary host fish included the eastern mosquitofish (Gambusia holbrooki), guppy (Poecilia reticulata) and bluegill (Lepomis macrochirus). The glochidial morphology of L. subangulata was similar to other lampsiline species. Using quantitative survey methods, no evidence of recent juvenile recruitment was found in the largest known extant L. subangulata population.