In situ detection of the Clostridium botulinum type C1 toxin gene in wetland sediments with a nested PCR assay

Applied and Environmental Microbiology
By: , and 

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Abstract

A nested PCR was developed for detection of the Clostridium botulinum type C1 toxin gene in sediments collected from wetlands where avian botulism outbreaks had or had not occurred. The C1 toxin gene was detected in 16 of 18 sites, demonstrating both the ubiquitous distribution of C. botulinum type C in wetland sediments and the sensitivity of the detection assay.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title In situ detection of the Clostridium botulinum type C1 toxin gene in wetland sediments with a nested PCR assay
Series title Applied and Environmental Microbiology
Volume 65
Issue 7
Year Published 1999
Language English
Publisher American Society for Microbiology
Contributing office(s) National Wildlife Health Center
Description 4 p.
First page 3240
Last page 3243