In situ detection of the Clostridium botulinum type C1 toxin gene in wetland sediments with a nested PCR assay
Applied and Environmental Microbiology
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Abstract
A nested PCR was developed for detection of the Clostridium botulinum type C1 toxin gene in sediments collected from wetlands where avian botulism outbreaks had or had not occurred. The C1 toxin gene was detected in 16 of 18 sites, demonstrating both the ubiquitous distribution of C. botulinum type C in wetland sediments and the sensitivity of the detection assay.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | In situ detection of the Clostridium botulinum type C1 toxin gene in wetland sediments with a nested PCR assay |
| Series title | Applied and Environmental Microbiology |
| DOI | 10.1128/AEM.65.7.3240-3243.1999 |
| Volume | 65 |
| Issue | 7 |
| Year Published | 1999 |
| Language | English |
| Publisher | American Society for Microbiology |
| Contributing office(s) | National Wildlife Health Center |
| Description | 4 p. |
| First page | 3240 |
| Last page | 3243 |
| Google Analytic Metrics | Metrics page |