Geochemical data indicate that the Springfield Plateau aquifer, a carbonate aquifer of the Ozark Plateaus Province in central USA, has two distinct hydrochemical zones. Within each hydrochemical zone, water from springs is geochemically and isotopically different than water from wells. Geochemical data indicate that spring water generally interacts less with the surrounding rock and has a shorter residence time, probably as a result of flowing along discrete fractures and solution openings, than water from wells. Water type throughout most of the aquifer was calcium bicarbonate, indicating that carbonate-rock dissolution is the primary geochemical process occurring in the aquifer. Concentrations of calcium, bicarbonate, dissolved oxygen and tritium indicate that most ground water in the aquifer recharged rapidly and is relatively young (less than 40 years). In general, field-measured properties, concentrations of many chemical constituents, and calcite saturation indices were greater in samples from the northern part of the aquifer (hydrochemical zone A) than in samples from the southern part of the aquifer (hydrochemical zone B). Factors affecting differences in the geochemical composition of ground water between the two zones are difficult to identify, but could be related to differences in chert content and possibly primary porosity, solubility of the limestone, and amount and type of cementation between zone A than in zone B. In addition, specific conductance, pH, alkalinity, concentrations of many chemical constituents and calcite saturation indices were greater in samples from wells than in samples from springs in each hydrochemical zone. In contrast, concentrations of dissolved oxygen, nitrite plus nitrate, and chloride generally were greater in samples from springs than in samples from wells. Water from springs generally flows rapidly through large conduits with minimum water-rock interactions. Water from wells flow through small fractures, which restrict flow and increase water-rock interactions. As a result, springs tend to be more susceptible to surface contamination than wells. The results of this study have important implications for the geochemical and hydrogeological processes of similar carbonate aquifers in other geographical locations. Copyright (C) 2000 John Wiley and Sons, Ltd.Geochemical data indicate that the Springfield Plateau carbonate aquifer has two distinct hydrochemical zones. With each hydrochemical zone, water from springs is geochemically and isotopically different from the water from wells. Spring water generally interacts less with the surrounding rock and has a shorter residence time, probably as a result of flowing along discrete fractures and solution openings, than water from wells. Factors affecting the differences in the geochemical composition of groundwater between the two zones are difficult to identify, but could be related to differences in chert content and possibly primary porosity, solubility of the limestone, and amount and type of cementation between zones.