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Gill Na+-K+-2Cl- cotransporter abundance and location in Atlantic salmon: Effects of seawater and smolting

American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

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Abstract

Na+-K+-2Clcotransporter abundance and location was examined in the gills of Atlantic salmon (Salmo salar) during seawater acclimation and smolting. Western blots revealed three bands centered at 285, 160, and 120 kDa. The Na+-K+-2Clcotransporter was colocalized with Na+-K+-ATPase to chloride cells on both the primary filament and secondary lamellae. Parr acclimated to 30 parts per thousand seawater had increased gill Na+-K+-2Cl cotransporter abundance, large and numerous Na+-K+-2Cl cotransporter immunoreactive chloride cells on the primary filament, and reduced numbers on the secondary lamellae. Gill Na+-K+-2Cl cotransporter levels were low in presmolts (February) and increased 3.3-fold in smolts (May), coincident with elevated seawater tolerance. Cotransporter levels decreased below presmolt values in postsmolts in freshwater (June). The size and number of immunoreactive chloride cells on the primary filament increased threefold during smolting and decreased in postsmolts. Gill Na+-K+-ATPase activity and Na+-K+-2Cl cotransporter abundance increased in parallel during both seawater acclimation and smolting. These data indicate a direct role of the Na+-K+-2Cl cotransporter in salt secretion by gill chloride cells of teleost fish.

Additional publication details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Gill Na+-K+-2Cl- cotransporter abundance and location in Atlantic salmon: Effects of seawater and smolting
Series title:
American Journal of Physiology - Regulatory, Integrative and Comparative Physiology
Volume:
280
Issue:
6
Year Published:
2001
Language:
English
Publisher:
American Physiological Society
Contributing office(s):
Leetown Science Center
Description:
9 p.
First page:
R1844
Last page:
R1852