Identification of methyl halide-utilizing genes in strain IMB-1, a methyl bromide-utilizing bacterium suggests a high degree of conservation of methyl halide-specific genes in gram-negative bacteria

Applied and Environmental Microbiology
By: , and 

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Abstract

Strain IMB-1, an aerobic methylotrophic member of the alpha subgroup of the Proteobacteria, can grow with methyl bromide as a sole carbon and energy source. A single cmu gene cluster was identified in IMB-1 that contained six open reading frames: cmuC, cmuA, orf146, paaE, hutI, and partial metF. CmuA from IMB-1 has high sequence homology to the methyltransferase CmuA from Methylobacterium chloromethanicum and Hyphomicrobium chloromethanicum and contains a C-terminal corrinoid-binding motif and an N-terminal methyl-transferase motif. However, cmuB, identified in M. chloromethanicum and H. chloromethanicum, was not detected in IMB-1.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Identification of methyl halide-utilizing genes in strain IMB-1, a methyl bromide-utilizing bacterium suggests a high degree of conservation of methyl halide-specific genes in gram-negative bacteria
Series title Applied and Environmental Microbiology
DOI 10.1128/AEM.67.4.1959-1963.2001
Volume 67
Issue 4
Year Published 2001
Language English
Publisher ASM
Contributing office(s) Toxic Substances Hydrology Program
Description 5 p.
First page 1959
Last page 1963