A rapid and efficient assay for extracting DNA from fungi
Letters in Applied Microbiology
By: Dale W. Griffin, C.A. Kellogg, K.K. Peak, and E.A. Shinn
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Abstract
Aims: A method for the rapid extraction of fungal DNA from small quantities of tissue in a batch-processing format was investigated. Methods and Results: Tissue (< 3.0 mg) was scraped from freshly-grown fungal isolates. The tissue was suspended in buffer AP1 and subjected to seven rounds of freeze/thaw using a crushed dry ice/ethanol bath and a boiling water bath. After a 30 min boiling step, the tissue was quickly ground against the wall of the microfuge tube using a sterile pipette tip. The Qiagen DNeasy Plant Tissue Kit protocol was then used to purify the DNA for PCR/ sequencing applications. Conclusions: The method allowed batch DNA extraction from multiple fungal isolates using a simple yet rapid and reliable assay. Significance and Impact of the Study: Use of this assay will allow researchers to obtain DNA from fungi quickly for use in molecular assays that previously required specialized instrumentation, was time-consuming or was not conducive to batch processing.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | A rapid and efficient assay for extracting DNA from fungi |
| Series title | Letters in Applied Microbiology |
| DOI | 10.1046/j.1472-765x.2002.01071.x |
| Volume | 34 |
| Issue | 3 |
| Year Published | 2002 |
| Language | English |
| Larger Work Type | Article |
| Larger Work Subtype | Journal Article |
| Larger Work Title | Letters in Applied Microbiology |
| First page | 210 |
| Last page | 214 |
| Google Analytic Metrics | Metrics page |