We present new ⁴⁰Ar/³⁹Ar ages for hornblende, muscovite, and biotite from metamorphic and plutonic rocks from the Yukon–Tanana Upland, Alaska. Integration of our data with published ⁴⁰Ar/³⁹Ar, kinematic, and metamorphic pressure (P) and temperature (T) data confirms and refines the complex interaction of metamorphism and tectonism proposed for the region. The oldest metamorphic episode(s) postdates Middle Permian magmatism and predates the intrusion of Late Triassic (215–212 Ma) granitoids into the Fortymile River assemblage (Taylor Mountain assemblage of previous papers). In the eastern Eagle quadrangle, rapid and widespread Early Jurassic cooling is indicated by ~188–186 Ma ⁴⁰Ar/³⁹Ar plateau ages for hornblende from plutons that intrude the Fortymile River assemblage, and for metamorphic minerals from the Fortymile River assemblage and the structurally underlying Nasina assemblage. We interpret these Early Jurassic ages to represent cooling resulting from northwest-directed contraction that emplaced the Fortymile River assemblage onto the Nasina assemblage to the north as well as the Lake George assemblage to the south. This cooling was the final stage of a continuum of subduction-related contraction that produced crustal thickening, intermediate- to high-P metamorphism within both the Fortymile River assemblage and the structurally underlying Lake George assemblage, and Late Triassic and Early Jurassic plutonism in the Fortymile River and Nasina assemblages. Although a few metamorphic samples from the Lake George assemblage yield Jurassic ⁴⁰Ar/³⁹Ar cooling ages, most yield Early Cretaceous ⁴⁰Ar/³⁹Ar ages: hornblende ~135–115 Ma, and muscovite and biotite ~110–108 Ma. We interpret the Early Cretaceous metamorphic cooling, in most areas, to have resulted from regional extension and exhumation of the lower plate, previously tectonically thickened during Early Jurassic and older convergence.