Aspects of the reproduction, age, growth, fish hosts, and culture of juveniles were determined for the endangered fanshell pearlymussel, Cyprogenia stegaria Rafinesque, 1820, in the Clinch River, Tennessee. Glochidia of C. stegaria are contained in red, worm-like conglutinates that resemble oligochaetes. Conglutinates are 20 to 80 mm long and are released through the excurrent aperture. Estimated fecundity was 22,357 to 63,459 glochidia/mussel. Eighty-four valves of C. stegaria were thin-sectioned for aging; ages ranged from 6 to 26 y. Of 16 fish species tested, 9 hosts were identified through induced infestations of glochidia: mottled sculpin (Cottus bairdi), banded sculpin (Cottus carolinae), greenside darter (Etheostoma blennioides), snubnose darter (Etheostoma simoterum), banded darter (Etheostoma zonale), tangerine darter (Percina aurantiaca), blotchside logperch (Percina burtoni), logperch (Percina caprodes), and Roanoke darter (Percina roanoka). Newly metamorphosed juveniles were cultured in recirculating and nonrecirculating aquaculture systems within dishes containing sediments of 300 to 500 μm diameter (sand) or <105 μm diameter (silt), and fed either the green algae Neochloris oleoabundans or Scenedesmus quadricauda daily. Growth and survival of juvenile mussels were highest in the nonrecirculating aquaculture system, with a mean survival of 72% after 2 wk and 38% after 4 wk.