
Toxicity of o,p′-DDE to medaka d-rR strain after a one-time embryonic exposure by in ovo nanoinjection: An early through juvenile life cycle assessment
Chemosphere
- By:
-
Sergio A. Villalobos
,
Diana M. Papoulias
,
Stephanie D. Pastva
,
Alan L. Blankenship
,
John C. Meadows
,
Donald E. Tillitt
, and John P. Giesy
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Abstract
The toxicity of o,p′-DDE (1,1-dichloro-2-(p-chlorophenyl)-2-(o-chlorophenyl) ethylene) was evaluated in embryos of medaka (Oryzias latipes) following a one time exposure via nanoinjection. Medaka eggs (early gastrula) were injected with 0.5 nl of triolein (vehicle control) or 0.5 nl of 4 graded doses (0.0005-0.5 ng/egg) of o,p′-DDE in triolein. Embryos were allowed to develop, and fry were reared. Embryonic survival was monitored daily during the first 10 d until hatching and thereafter, on a weekly basis until day 59, at which time the fish were monitored for sexual maturity until day 107. In general, o,p′-DDE caused a dose- and time-dependent mortality. No changes in mortality were observed between the last two time points (day 38 and 59, respectively), and hence a 59 day-LD50 of 346 ng o,p′-DDE/egg was derived from the linear dose-response relationship. Prior to late stage death, only isolated cases of cardiovascular lesions and spinal deformities were observed, but were not dose-dependent. The lowest observable adverse effect level (LOAEL), based on upper 95% CI for regression line=0.0018 mg/kg, and the LOAEL based on exposure doses=0.5 mg/kg. Likewise, the no observable adverse effect level (NOAEL) based on linear extrapolation to 100% survival=0.0000388 mg/kg, while the NOAEL based on exposure doses=0.05 mg/kg. The nanoinjection medaka model has potential in the study of hormonally active compounds in the environment.
Additional publication details
- Publication type:
- Article
- Publication Subtype:
- Journal Article
- Title:
- Toxicity of o,p′-DDE to medaka d-rR strain after a one-time embryonic exposure by in ovo nanoinjection: An early through juvenile life cycle assessment
- Series title:
- Chemosphere
- DOI:
- 10.1016/S0045-6535(03)00583-6
- Volume:
- 53
- Issue:
- 8
- Year Published:
- 2003
- Language:
- English
- Publisher:
- Elsevier
- Description:
- 8 p.
- First page:
- 819
- Last page:
- 826
- Online Only (Y/N):
- N
- Additional Online Files (Y/N):
- N