Hair samples are an increasingly important DNA source for wildlife studies, yet optimal storage methods and DNA degradation rates have not been rigorously evaluated. We tested amplification success rates over a one-year storage period for DNA extracted from brown bear (Ursus arctos) hair samples preserved using silica desiccation and -20C freezing. For three nuclear DNA microsatellites, success rates decreased significantly after a six-month time point, regardless of storage method. For a 1000 bp mitochondrial fragment, a similar decrease occurred after a two-week time point. Minimizing delays between collection and DNA extraction will maximize success rates for hair-based noninvasive genetic sampling projects.
Additional publication details
|Publication Subtype||Journal Article|
|Title||A quantitative evaluation of two methods for preserving hair samples|
|Series title||Molecular Ecology Notes|
|Contributing office(s)||Northern Rocky Mountain Science Center|
|Online Only (Y/N)||N|
|Additional Online Files (Y/N)||N|