A study was implemented to test site preparation methods and artificial regeneration of three oak (Quercus spp.) species on four agricultural fields in the Lower Mississippi Alluvial Valley in Louisiana, USA. Six years after establishment, few consistent differences were found in oak density between sowing acorn methods (seed drill versus broadcast seeding), autumn sowing versus spring sowing, and sowing acorns versus planting oak seedlings. Results indicated that some degree of site preparation is needed to establish oak seedlings but few differences were found between site preparation treatments. These results indicate that no one prescription for oak regeneration fits all potential afforestation projects in the Lower Mississippi Alluvial Valley. Successful bottomland hardwood afforestation projects will require plans that include specific objectives, site evaluation, and a regeneration prescription prior to sowing the first seed or planting the first seedling.