Detection of Clostridium botulinum type C cells in the gastrointestinal tracts of Mozambique tilapia (Oreochromis mossambicus) by polymerase chain reaction

Journal of Wildlife Diseases
By: , and 

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Abstract

We established a method of directly detecting Clostridium botulinum type C cells, while minimizing spore detection, in the intestinal contents of Mozambique tilapia (Oreochromis mossambicus). This technique involved extraction of predominantly cellular DNA from tilapia intestinal tracts and used a polymerase chain reaction assay to detect presence of type C1 toxin gene. We consistently detected C. botulinum type C cells in tilapia gastrointestinal contents at a level of 7.5×104 cells per 0.25 g material or 1.9×103 cells. This technique is useful for determining prevalence of the potentially active organisms within a given population of fish and may be adapted to other types of C. botulinum and vertebrate populations as well.

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Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Detection of Clostridium botulinum type C cells in the gastrointestinal tracts of Mozambique tilapia (Oreochromis mossambicus) by polymerase chain reaction
Series title Journal of Wildlife Diseases
DOI 10.7589/0090-3558-40.4.749
Volume 40
Issue 4
Year Published 2004
Language English
Publisher Wildlife Disease Association
Contributing office(s) National Wildlife Health Center
Description 5 p.
First page 749
Last page 753
Country United States
State California
Other Geospatial Salton Sea
Online Only (Y/N) N
Additional Online Files (Y/N) N