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Detection of Clostridium botulinum type C cells in the gastrointestinal tracts of Mozambique tilapia (Oreochromis mossambicus) by polymerase chain reaction

Journal of Wildlife Diseases

By:
, , , and
https://doi.org/10.7589/0090-3558-40.4.749

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Abstract

We established a method of directly detecting Clostridium botulinum type C cells, while minimizing spore detection, in the intestinal contents of Mozambique tilapia (Oreochromis mossambicus). This technique involved extraction of predominantly cellular DNA from tilapia intestinal tracts and used a polymerase chain reaction assay to detect presence of type C1 toxin gene. We consistently detected C. botulinum type C cells in tilapia gastrointestinal contents at a level of 7.5×104 cells per 0.25 g material or 1.9×103 cells. This technique is useful for determining prevalence of the potentially active organisms within a given population of fish and may be adapted to other types of C. botulinum and vertebrate populations as well.

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Additional publication details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Detection of Clostridium botulinum type C cells in the gastrointestinal tracts of Mozambique tilapia (Oreochromis mossambicus) by polymerase chain reaction
Series title:
Journal of Wildlife Diseases
DOI:
10.7589/0090-3558-40.4.749
Volume:
40
Issue:
4
Year Published:
2004
Language:
English
Publisher:
Wildlife Disease Association
Contributing office(s):
National Wildlife Health Center
Description:
5 p.
First page:
749
Last page:
753
Country:
United States
State:
California
Other Geospatial:
Salton Sea
Online Only (Y/N):
N
Additional Online Files (Y/N):
N