Population structure of Columbia spotted frogs (Rana luteiventris) is strongly affected by the landscape

Molecular Ecology
By: , and 



Landscape features such as mountains, rivers, and ecological gradients may strongly affect patterns of dispersal and gene flow among populations and thereby shape population dynamics and evolutionary trajectories. The landscape may have a particularly strong effect on patterns of dispersal and gene flow in amphibians because amphibians are thought to have poor dispersal abilities. We examined genetic variation at six microsatellite loci in Columbia spotted frogs (Rana luteiventris) from 28 breeding ponds in western Montana and Idaho, USA, in order to investigate the effects of landscape structure on patterns of gene flow. We were particularly interested in addressing three questions: (i) do ridges act as barriers to gene flow? (ii) is gene flow restricted between low and high elevation ponds? (iii) does a pond equal a 'randomly mating population' (a deme)? We found that mountain ridges and elevational differences were associated with increased genetic differentiation among sites, suggesting that gene flow is restricted by ridges and elevation in this species. We also found that populations of Columbia spotted frogs generally include more than a single pond except for very isolated ponds. There was also evidence for surprisingly high levels of gene flow among low elevation sites separated by large distances. Moreover, genetic variation within populations was strongly negatively correlated with elevation, suggesting effective population sizes are much smaller at high elevation than at low elevation. Our results show that landscape features have a profound effect on patterns of genetic variation in Columbia spotted frogs.

Study Area

Publication type Article
Publication Subtype Journal Article
Title Population structure of Columbia spotted frogs (Rana luteiventris) is strongly affected by the landscape
Series title Molecular Ecology
DOI 10.1111/j.1365-294X.2005.02426.x
Volume 14
Issue 2
Year Published 2005
Language English
Publisher Blackwell Science
Publisher location Oxford
Contributing office(s) Northern Rocky Mountain Science Center
Description 14 p.
First page 483
Last page 496
Country United States
State Idaho, Montana
Online Only (Y/N) N
Additional Online Files (Y/N) N
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