A method for measuring total thiaminase activity in fish tissues

Journal of Aquatic Animal Health
By: , and 



An accurate, quantitative, and rapid method for the measurement of thiaminase activity in fish samples is required to provide sufficient information to characterize the role of dietary thiaminase in the onset of thiamine deficiency in Great Lakes salmonines. A radiometric method that uses 14C-thiamine was optimized for substrate and co-substrate (nicotinic acid) concentrations, incubation time, and sample dilution. Total thiaminase activity was successfully determined in extracts of selected Great Lakes fishes and invertebrates. Samples included whole-body and selected tissues of forage fishes. Positive control material prepared from frozen alewives Alosa pseudoharengus collected in Lake Michigan enhanced the development and application of the method. The method allowed improved discrimination of thiaminolytic activity among forage fish species and their tissues. The temperature dependence of the thiaminase activity observed in crude extracts of Lake Michigan alewives followed a Q10 = 2 relationship for the 1-37??C temperature range, which is consistent with the bacterial-derived thiaminase I protein. ?? Copyright by the American Fisheries Society 2005.
Publication type Article
Publication Subtype Journal Article
Title A method for measuring total thiaminase activity in fish tissues
Series title Journal of Aquatic Animal Health
DOI 10.1577/H03-083.1
Volume 17
Issue 1
Year Published 2005
Language English
Publisher Taylor & Francis
Contributing office(s) Columbia Environmental Research Center
Description 13 p.
First page 82
Last page 94
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