Application of Bacteroides fragilis phage as an alternative indicator of sewage pollution in Tampa Bay, Florida

Estuaries and Coasts
By:  and 



Traditional fecal coliform bacterial indicators have been found to be severely limited in determining the significance and sources of fecal contamination in ambient waters of tropical and subtropical regions. The bacteriophages that infectBacteroides fragilis have been suggested as better fecal indicators and at least one type may be human specific. In this study, the phages that infectB. fragilis host RYC2056 (RYC), including phage B56-3, and host ATCC 51477-HSP40 (HSP), including the human specific phage B40-8, were evaluated in the drainage basins of Tampa Bay, 7 samples (n=62), or 11%, tested positive for the presence of phages infecting the host HSP, whereas 28 samples, or 45%, tested positive using the host RYC. A survival study was also done to compare the persistence of phages B56-3 and B40-8 to MS2 coliphage in seawater at various temperatures. The decay rates for MS2 were 0.239 log10 d−1 at 10°C, but increased to 0.896 at 20°C and 2.62 log10 d−1 at 30°C. The twoB. fragilis phages persisted much longer in the seawater compared to the coliphage and showed little variation between the temperatures. All sewage influents sampled from area wastewater treatment plants contained phages that infected the twoB. fragilis hosts at levels from 1.2×104 to 1.11×105 pfu 100 ml−1 for host RYC and 67 to 350 pfu 100 ml−1 for host HSP. Of the 7 chlorinated effluent samples tested, 3 were positive for the presence of the phage using the host RYC and the phage enrichment method, with levels estimated to be <10 pfu 100 ml−1. No phages were detected using the host HSP in the treated sewage effluent. Coliphages were found in 3 of the 7 effluent samples at a range of 30 to 1.2×103 pfu 100 ml−1.

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Publication type Article
Publication Subtype Journal Article
Title Application of Bacteroides fragilis phage as an alternative indicator of sewage pollution in Tampa Bay, Florida
Series title Estuaries and Coasts
DOI 10.1007/BF02781993
Volume 29
Issue 2
Year Published 2006
Language English
Publisher Springer
Description 11 p.
First page 246
Last page 256
Country United States
State Florida
Other Geospatial Tampa Bay
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