We evaluated the use of a simple rake sampling technique for predicting the biomass of submersed aquatic vegetation. Vegetation sampled from impounded areas of the Mississippi River using a rake sampling technique, was compared with vegetation harvested from 0.33-m2 quadrats. The resulting data were used to model the relationship between rake indices and vegetation biomass (total and for individual species). We constructed linear regression models using log-transformed biomass data for sites sampled in 1999 and 2000. Data collected in 2001 were used to validate the resulting models. The coefficient of determination (R 2) for predicting total biomass was 0.82 and ranged from 0.59 (Potamogeton pectinatus) to 0.89 (Ceratophyllum demersum) for individual species. Application of the model to estimate total submersed aquatic vegetation is illustrated using data collected independent of this study. The accuracy and precision of the models tested indicate that the rake method data may be used to predict total vegetation biomass and biomass of selected species; however, the method should be tested in other regions, in other plant communities, and on other species. ?? 2006 Springer Science+Business Media B.V.