Rapid polymerase chain reaction diagnosis of white-nose syndrome in bats
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Abstract
A newly developed polymerase chain reaction (PCR)-based method to rapidly and specifically detect Geomyces destructans on the wings of infected bats from small quantities (1–2 mg) of tissue is described in the current study (methods for culturing and isolating G. destructans from bat skin are also described). The lower limits of detection for PCR were 5 fg of purified fungal DNA or 100 conidia per 2 mg of wing tissue. By using histology as the standard, the PCR had a diagnostic specificity of 100% and a diagnostic sensitivity of 96%, whereas the diagnostic sensitivity of culture techniques was only 54%. The accuracy and fast turnaround time of PCR provides field biologists with valuable information on infection status more rapidly than traditional methods, and the small amount of tissue required for the test would allow diagnosis of white-nose syndrome in live animals.
Study Area
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Rapid polymerase chain reaction diagnosis of white-nose syndrome in bats |
| Series title | Journal of Veterinary Diagnostic Investigation |
| DOI | 10.1177/104063871002200208 |
| Volume | 22 |
| Issue | 2 |
| Year Published | 2010 |
| Language | English |
| Publisher | American Association of Veterinary Laboratory Diagnosticians |
| Contributing office(s) | National Wildlife Health Center |
| Description | 7 p. |
| First page | 224 |
| Last page | 230 |
| Country | United States |
| State | Alabama, Connecticut, Indiana, Maryland, New Hampshire, New Jersey, New York, Ohio, Pennsylvania, Virginia, Vermont, West Virginia, Wisconsin |
| Online Only (Y/N) | N |
| Additional Online Files (Y/N) | N |
| Google Analytic Metrics | Metrics page |