Even though nitrogen (N) is a key nutrient for successful cranberry production, N cycling in cranberry agroecosystems is not completely understood. Prior research has focused mainly on timing and uptake of ammonium fertilizer, but the objective of our study was to evaluate the potential for additional N contributions from hydrologic inputs (flooding, irrigation, groundwater, and precipitation) and organic matter (OM). Plant biomass, soil, surface and groundwater samples were collected from five cranberry beds (cranberry production fields) on four different farms, representing both upland and lowland systems. Estimated average annual plant uptake (63.3 ± 22.5 kg N ha⁻¹ year⁻¹) exceeded total average annual fertilizer inputs (39.5 ± 11.6 kg N ha⁻¹ year⁻¹). Irrigation, precipitation, and floodwater N summed to an average 23 ± 0.7 kg N ha⁻¹ year⁻¹, which was about 60% of fertilizer N. Leaf and stem litterfall added 5.2 ± 1.2 and 24.1 ± 3.0 kg N ha⁻¹ year⁻¹ respectively. The estimated net N mineralization rate from the buried bag technique was 5 ± 0.2 kg N ha⁻¹ year⁻¹, which was nearly 15% of fertilizer N. Dissolved organic nitrogen represented a significant portion of the total N pool in both surface water and soil samples. Mixed-ion exchange resin core incubations indicated that 80% of total inorganic N from fertilizer, irrigation, precipitation, and mineralization was nitrate, and approximately 70% of recovered inorganic N from groundwater was nitrate. There was a weak but significant negative relationship between extractable soil ammonium concentrations and ericoid mycorrhizal colonization (ERM) rates (r = −0.22, P < 0.045). Growers may benefit from balancing the N inputs from hydrologic sources and OM relative to fertilizer N in order to maximize the benefits of ERM fungi in actively mediating N cycling in cranberry agroecosystems.