Quantitative polymerase chain reaction (PCR) for detection of aquatic animal pathogens in a diagnostic laboratory setting

Journal of Aquatic Animal Health
By: , and 

Links

Abstract

Real-time, or quantitative, polymerase chain reaction (qPCR) is quickly supplanting other molecular methods for detecting the nucleic acids of human and other animal pathogens owing to the speed and robustness of the technology. As the aquatic animal health community moves toward implementing national diagnostic testing schemes, it will need to evaluate how qPCR technology should be employed. This review outlines the basic principles of qPCR technology, considerations for assay development, standards and controls, assay performance, diagnostic validation, implementation in the diagnostic laboratory, and quality assurance and control measures. These factors are fundamental for ensuring the validity of qPCR assay results obtained in the diagnostic laboratory setting.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Quantitative polymerase chain reaction (PCR) for detection of aquatic animal pathogens in a diagnostic laboratory setting
Series title Journal of Aquatic Animal Health
DOI 10.1080/08997659.2011.620217
Volume 23
Issue 3
Year Published 2011
Language English
Publisher Taylor & Francis
Contributing office(s) Western Fisheries Research Center
Description 14 p.
Larger Work Type Article
Larger Work Subtype Journal Article
Larger Work Title Journal of Aquatic Animal Health
First page 148
Last page 161