Persistence of DNA in carcasses, slime and avian feces may affect interpretation of environmental DNA data

PLoS ONE
By: , and 

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Abstract

The prevention of non-indigenous aquatic invasive species spreading into new areas is a goal of many resource managers. New techniques have been developed to survey for species that are difficult to capture with conventional gears that involve the detection of their DNA in water samples (eDNA). This technique is currently used to track the invasion of bigheaded carps (silver carp and bighead carp; Hypophthalmichthys molitrix and H. nobilis) in the Chicago Area Waterway System and Upper Mississippi River. In both systems DNA has been detected from silver carp without the capture of a live fish, which has led to some uncertainty about the source of the DNA. The potential contribution to eDNA by vectors and fomites has not been explored. Because barges move from areas with a high abundance of bigheaded carps to areas monitored for the potential presence of silver carp, we used juvenile silver carp to simulate the barge transport of dead bigheaded carp carcasses, slime residue, and predator feces to determine the potential of these sources to supply DNA to uninhabited waters where it could be detected and misinterpreted as indicative of the presence of live bigheaded carp. Our results indicate that all three vectors are feasible sources of detectable eDNA for at least one month after their deposition. This suggests that current monitoring programs must consider alternative vectors of DNA in the environment and consider alternative strategies to minimize the detection of DNA not directly released from live bigheaded carps.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Persistence of DNA in carcasses, slime and avian feces may affect interpretation of environmental DNA data
Series title PLoS ONE
DOI 10.1371/journal.pone.0113346
Volume 9
Issue 11
Year Published 2014
Language English
Publisher Public Library of Science
Contributing office(s) Upper Midwest Environmental Sciences Center
Description e113346; 7 p.
Online Only (Y/N) Y
Additional Online Files (Y/N) N