Development of eighteen microsatellite loci in walleye (Sander vitreus)

Conservation Genetics Resources
By: , and 

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Abstract

A suite of tri- and tetra-nucleotide microsatellite loci were developed for walleye (Sander vitreus) from 454 pyrosequencing data. Eighteen of the 50 primer sets tested amplified consistently in 35 walleye from two lakes on Isle Royale, Lake Superior: Chickenbone Lake and Whittlesey Lake. The loci displayed moderate levels of allelic diversity (average 5.5 alleles/locus) and heterozygosity (average 35.8 %). Levels of genetic diversity were sufficient to produce unique multi-locus genotypes and detect phylogeographic structuring as individuals assigned back to their population of origin. Cross-species amplification within S. canadensis(sauger) was successful for 15 loci, and 11 loci were diagnostic to species. The loci characterized here will be useful for detecting fine-scale spatial structuring, resolving the taxonomic status of Sander species and sub-species, and detecting walleye/sauger hybrids.

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Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Development of eighteen microsatellite loci in walleye (Sander vitreus)
Series title Conservation Genetics Resources
DOI 10.1007/s12686-014-0275-8
Volume 6
Issue 4
Year Published 2014
Language English
Publisher Springer
Contributing office(s) Leetown Science Center
Description 3 p.
First page 1019
Last page 1021
Country United States
State Michigan
Other Geospatial Chickenbone Lake;Whittlesey Lake
Online Only (Y/N) N
Additional Online Files (Y/N) N