Development of eighteen microsatellite loci in walleye (Sander vitreus)
Links
- More information: Publisher Index Page (via DOI)
- Download citation as: RIS | Dublin Core
Abstract
A suite of tri- and tetra-nucleotide microsatellite loci were developed for walleye (Sander vitreus) from 454 pyrosequencing data. Eighteen of the 50 primer sets tested amplified consistently in 35 walleye from two lakes on Isle Royale, Lake Superior: Chickenbone Lake and Whittlesey Lake. The loci displayed moderate levels of allelic diversity (average 5.5 alleles/locus) and heterozygosity (average 35.8 %). Levels of genetic diversity were sufficient to produce unique multi-locus genotypes and detect phylogeographic structuring as individuals assigned back to their population of origin. Cross-species amplification within S. canadensis(sauger) was successful for 15 loci, and 11 loci were diagnostic to species. The loci characterized here will be useful for detecting fine-scale spatial structuring, resolving the taxonomic status of Sander species and sub-species, and detecting walleye/sauger hybrids.
Study Area
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Development of eighteen microsatellite loci in walleye (Sander vitreus) |
| Series title | Conservation Genetics Resources |
| DOI | 10.1007/s12686-014-0275-8 |
| Volume | 6 |
| Issue | 4 |
| Year Published | 2014 |
| Language | English |
| Publisher | Springer |
| Contributing office(s) | Leetown Science Center |
| Description | 3 p. |
| First page | 1019 |
| Last page | 1021 |
| Country | United States |
| State | Michigan |
| Other Geospatial | Chickenbone Lake;Whittlesey Lake |
| Online Only (Y/N) | N |
| Additional Online Files (Y/N) | N |
| Google Analytic Metrics | Metrics page |