Direct detection of fungal siderophores on bats with white-nose syndrome via fluorescence microscopy-guided ambient ionization mass spectrometry

PLoS ONE
By: , and 

Links

Abstract

White-nose syndrome (WNS) caused by the pathogenic fungus Pseudogymnoascus destructans is decimating the populations of several hibernating North American bat species. Little is known about the molecular interplay between pathogen and host in this disease. Fluorescence microscopy ambient ionization mass spectrometry was used to generate metabolic profiles from the wings of both healthy and diseased bats of the genus Myotis. Fungal siderophores, molecules that scavenge iron from the environment, were detected on the wings of bats with WNS, but not on healthy bats. This work is among the first examples in which microbial molecules are directly detected from an infected host and highlights the ability of atmospheric ionization methodologies to provide direct molecular insight into infection.

Study Area

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Direct detection of fungal siderophores on bats with white-nose syndrome via fluorescence microscopy-guided ambient ionization mass spectrometry
Series title PLoS ONE
DOI 10.1371/journal.pone.0119668
Volume 10
Issue 3
Year Published 2015
Language English
Publisher PLOS
Contributing office(s) National Wildlife Health Center
Description e0119668: 12 p.
Country United States
State Pennsylvania, West Virginia
Online Only (Y/N) N
Additional Online Files (Y/N) N