Cross-species amplification of microsatellite markers in the Great Horned Owl Bubo virginianus, Short-eared Owl Asio flammeus and Snowy Owl B. scandiacus for use in population genetics, individual identification and parentage studies
Journal of the Yamashina Institute for Ornithology
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Abstract
Using DNA from blood and feathers, we screened twenty-four microsatellite primer pairs initially developed for six strigid owls, and four primer pairs shown to be polymorphic across avian taxa, for their utility in Great Horned Owl (Bubo virginianus), Short-eared Owl (Asio flammeus), and Snowy Owl (Bubo scandiacus). Eight of these primers reliably amplified polymorphic fragments in Great Horned Owl, eleven in Short-eared owl, and ten in Snowy Owl. Analyses of results from presumably unrelated owls demonstrate the utility of these loci for individual identification, parentage assignment, and population genetics studies.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Cross-species amplification of microsatellite markers in the Great Horned Owl Bubo virginianus, Short-eared Owl Asio flammeus and Snowy Owl B. scandiacus for use in population genetics, individual identification and parentage studies |
| Series title | Journal of the Yamashina Institute for Ornithology |
| DOI | 10.3312/jyio.44.1 |
| Volume | 44 |
| Issue | 1 |
| Year Published | 2012 |
| Language | English |
| Publisher | Yamashina Institute for Ornithology |
| Publisher location | Tokyo |
| Contributing office(s) | Alaska Science Center Biology WTEB |
| Description | 12 p. |
| First page | 1 |
| Last page | 12 |
| Online Only (Y/N) | N |
| Additional Online Files (Y/N) | N |
| Google Analytic Metrics | Metrics page |