Development of 20 TaqMan assays differentiating the endangered shortnose and Lost River suckers

Conservation Genetics Resources
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Abstract

Accurate species identification is vital to conservation and management of species at risk. Species identification is challenging when taxa express similar phenotypic characters and form hybrids, for example the endangered shortnose sucker (Chasmistes brevirostris) and Lost River sucker (Deltistes luxatus). Here, we developed 20 Taqman assays that differentiate these species (19 nuclear DNA and one mitochondrial DNA). Assays were evaluated in 160 young-of-the-year identified to species using meristic counts. Alleles were not fixed between species, but species were highly differentiated (F ST = 0.753, P < 0.001). The assays developed herein will be a valuable tool for resource managers.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Development of 20 TaqMan assays differentiating the endangered shortnose and Lost River suckers
Series title Conservation Genetics Resources
DOI 10.1007/s12686-015-0474-y
Volume 7
Issue 3
Year Published 2015
Language English
Publisher Springer
Publisher location Netherlands
Contributing office(s) Western Fisheries Research Center
Description 4 p.
First page 673
Last page 676
Online Only (Y/N) N
Additional Online Files (Y/N) N