Development of 20 TaqMan assays differentiating the endangered shortnose and Lost River suckers
Conservation Genetics Resources
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Abstract
Accurate species identification is vital to conservation and management of species at risk. Species identification is challenging when taxa express similar phenotypic characters and form hybrids, for example the endangered shortnose sucker (Chasmistes brevirostris) and Lost River sucker (Deltistes luxatus). Here, we developed 20 Taqman assays that differentiate these species (19 nuclear DNA and one mitochondrial DNA). Assays were evaluated in 160 young-of-the-year identified to species using meristic counts. Alleles were not fixed between species, but species were highly differentiated (F ST = 0.753, P < 0.001). The assays developed herein will be a valuable tool for resource managers.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Development of 20 TaqMan assays differentiating the endangered shortnose and Lost River suckers |
| Series title | Conservation Genetics Resources |
| DOI | 10.1007/s12686-015-0474-y |
| Volume | 7 |
| Issue | 3 |
| Year Published | 2015 |
| Language | English |
| Publisher | Springer |
| Publisher location | Netherlands |
| Contributing office(s) | Western Fisheries Research Center |
| Description | 4 p. |
| First page | 673 |
| Last page | 676 |
| Online Only (Y/N) | N |
| Additional Online Files (Y/N) | N |
| Google Analytic Metrics | Metrics page |