Investigations of novel unsaturated bile salts of male sea lamprey as potential chemical cues
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Abstract
Sulfated bile salts function as chemical cues that coordinate reproduction in sea lamprey, Petromyzon marinus. 7α, 12α, 24-trihydroxy-5α-cholan-3-one 24-sulfate (3kPZS) is the most abundant known bile salt released by sexually mature male sea lampreys and attracts ovulated females. However, previous studies showed that the male-produced pheromone consists of unidentified components in addition to 3kPZS. Here, analysis of water conditioned with mature male sea lampreys indicated the presence of 4 oxidized, unsaturated compounds with molecular weights of 466 Da, 468 Da, and 2 of 470 Da. These compounds were not detectable in water conditioned with immature male sea lampreys. By using mass spectrometry, 4 A-ring unsaturated sulfated bile salts were tentatively identified from male washings as 2 4-ene, a 1-ene, and a 1,4-diene analogs. These were synthesized to determine if they attracted ovulated female sea lampreys to spawning nests in natural streams. One of the novel synthetic bile salts, 3 keto-1-ene PZS, attracted ovulated females to the point of application at a concentration of 10-12 M. This study reveals the structural diversity of bile salts in sea lamprey, some of which have been demonstrated to be pheromonal cues.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Investigations of novel unsaturated bile salts of male sea lamprey as potential chemical cues |
| Series title | Journal of Chemical Ecology |
| DOI | 10.1007/s10886-014-0511-4 |
| Volume | 40 |
| Issue | 10 |
| Year Published | 2014 |
| Language | English |
| Publisher | Kluwer Academic |
| Publisher location | New York, NY |
| Contributing office(s) | Great Lakes Science Center |
| Description | 10 p. |
| First page | 1152 |
| Last page | 1160 |
| Online Only (Y/N) | N |
| Additional Online Files (Y/N) | N |
| Google Analytic Metrics | Metrics page |