Natural soil reservoirs for human pathogenic and fecal indicator bacteria

By: , and 

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Abstract

Soils receive inputs of human pathogenic and indicator bacteria through land application of animal manures or sewage sludge, and inputs by wildlife. Soil is an extremely heterogeneous substrate and contains meso- and macrofauna that may be reservoirs for bacteria of human health concern. The ability to detect and quantify bacteria of human health concern is important in risk assessments and in evaluating the efficacy of agricultural soil management practices that are protective of crop quality and protective of adjacent water resources. The present chapter describes the distribution of selected Gram-positive and Gram-negative bacteria in soils. Methods for detecting and quantifying soilborne bacteria including extraction, enrichment using immunomagnetic capture, culturing, molecular detection and deep sequencing of metagenomic DNA to detect pathogens are overviewed. Methods for strain phenotypic and genotypic characterization are presented, as well as how comparison with clinical isolates can inform the potential for human health risk.

Additional publication details

Publication type Book chapter
Publication Subtype Book Chapter
Title Natural soil reservoirs for human pathogenic and fecal indicator bacteria
DOI 10.1128/9781555818821.ch3.3.2
Year Published 2016
Language English
Publisher American Society for Microbiology
Contributing office(s) Great Lakes Science Center
Description 13 p.
Larger Work Type Book
Larger Work Title Manual of environmental microbiology
First page 3.3.2-1
Last page 3.3.2-12