Methane oxidation and molecular characterization of methanotrophs from a former mercury mine impoundment

By: , and 



The Herman Pit, once a mercury mine, is an impoundment located in an active geothermal area. Its acidic waters are permeated by hundreds of gas seeps. One seep was sampled and found to be composed of mostly CO2 with some CH4 present. The δ13CH4 value suggested a complex origin for the methane: i.e., a thermogenic component plus a biological methanogenic portion. The relatively 12C-enriched CO2 suggested a reworking of the ebullitive methane by methanotrophic bacteria. Therefore, we tested bottom sediments for their ability to consume methane by conducting aerobic incubations of slurried materials. Methane was removed from the headspace of live slurries, and subsequent additions of methane resulted in faster removal rates. This activity could be transferred to an artificial, acidic medium, indicating the presence of acidophilic or acid-tolerant methanotrophs, the latter reinforced by the observation of maximum activity at pH = 4.5 with incubated slurries. A successful extraction of sterol and hopanoid lipids characteristic of methanotrophs was achieved, and their abundances greatly increased with increased sediment methane consumption. DNA extracted from methane-oxidizing enrichment cultures was amplified and sequenced for pmoA genes that aligned with methanotrophic members of the Gammaproteobacteria. An enrichment culture was established that grew in an acidic (pH 4.5) medium via methane oxidation.

Study Area

Publication type Article
Publication Subtype Journal Article
Title Methane oxidation and molecular characterization of methanotrophs from a former mercury mine impoundment
Series title Microorganisms
DOI 10.3390/microorganisms3020290
Volume 3
Issue 2
Year Published 2015
Language English
Publisher MDPI AG
Publisher location Basel, Switzerland
Contributing office(s) National Research Program - Western Branch
Description 20 p.
First page 290
Last page 309
Country United States
State California
Other Geospatial Herman Mine
Online Only (Y/N) N
Additional Online Files (Y/N) N
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