Infectious hematopoietic necrosis virus detected by separation and incubation of cells from salmonid cavity fluid.

Canadian Journal of Fisheries and Aquatic Sciences
By:  and 

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Abstract

Infectious hematopoietic necrosis (IHN) virus is usually detected by inoculating susceptible cell cultures with cavity ("ovarian") fluid (CF) from spawning females. We identified additional adult carriers of virus in spawning populations of steelhead trout (Salmo gairdneri) and sockeye salmon (Oncorhynchus nerka) by collecting nonerythrocytic cells from CF samples by low-speed centrifugation, culturing the cells for at least 7 d at 15 °C, and then testing the culture medium for virus. Virus appeared in the cultured cells from some samples of CF that remained negative during incubation. In additional samples of CF from these species, the virus titer increased in cultured cells compared with the titer in the original CF sample. With chinook salmon (O.tshawytscha), no negative samples converted to positive during incubation, but the virus titer was retained in incubated CF cells, but not in cell-free CF.

Additional publication details

Publication type Article
Publication Subtype Journal Article
Title Infectious hematopoietic necrosis virus detected by separation and incubation of cells from salmonid cavity fluid.
Series title Canadian Journal of Fisheries and Aquatic Sciences
DOI 10.1139/f87-126
Volume 44
Issue 5
Year Published 1987
Language English
Publisher NRC Research Press
Contributing office(s) Western Fisheries Research Center, Alaska Science Center
Description 5 p.
First page 1071
Last page 1075