Comparison of five techniques for the detection of Renibacterium salmoninarum in adult coho salmon.
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Abstract
Samples of kidney, spleen, coelomic fluid, and blood from 56 sexually mature coho salmon Oncorhynchus kisutch were examined for infection by Renibacterium salmoninarum by five methods. The overall prevalence (all sample types combined) of R. salmoninarum in the fish was 100% by the enzyme-linked immunosorbent assay, 86% by the combined results of the direct fluorescent antibody and the direct filtration-fluorescent antibody techniques, 39% by culture, 11% by counterimmunoelectrophoresis, and 5% by agarose gel immunodiffusion. There was a significant positive correlation (P < 0.001) between the enzyme-linked immunosorbent assay absorbance levels and the counts by fluorescent antibody techniques for kidney, spleen, and coelomic fluid, and significant positive correlations (P < 0.001) in enzyme-linked immunosorbent assay absorbance levels for all four of the sample types.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Comparison of five techniques for the detection of Renibacterium salmoninarum in adult coho salmon. |
| Series title | Transactions of the American Fisheries Society |
| DOI | 10.1577/1548-8659(1987)116<882:COFTFT>2.0.CO;2 |
| Volume | 116 |
| Issue | 6 |
| Year Published | 1987 |
| Language | English |
| Publisher | American Fisheries Society |
| Contributing office(s) | Alaska Science Center, Western Fisheries Research Center |
| Description | 8 p. |
| First page | 882 |
| Last page | 890 |
| Google Analytic Metrics | Metrics page |